Journal: Bioactive Materials

Article Title: AdMSC spheroids encapsulating antioxidant hybrid protein carrier for irradiation-damaged salivary gland repair

doi: 10.1016/j.bioactmat.2026.03.049

Figure Lengend Snippet: In vivo retention and clearance kinetics of DiR-labeled AdMSC spheroids after injection into mouse salivary glands. (a) DiR-labeled AdMSC spheroids or spheroid + GC (untreated, spheroid, spheroid + GC 5% w/v, spheroid + GC 7% w/v) were injected into the IR-damaged mouse salivary gland, and their fluorescence signals were monitored by IVIS from days 1 to 28 until no detectable signal remained. (b) Quantification of the average radiant efficiency in the salivary gland region over time. (c) At day 28 post-injection, major organs (heart, liver, spleen, kidneys, lungs, and stomach) were harvested and imaged by IVIS to assess DiR signals. Data are presented as mean ± SD (n = 3 or n = 4). Statistical analysis was performed using one-way ANOVA followed by Tukey's post-hoc test. ns P > 0.05, ∗ P ≤ 0.05, ∗∗ P ≤ 0.01, and ∗∗∗ P ≤ 0.001.

Article Snippet: For in vivo fluorescence imaging, mice were anesthetized and scanned using an IVIS imaging system (IVIS Lumina S5, PerkinElmer) at predetermined time points (1, 3, 5, 7, 10, 14, 21, and 28 days) with excitation/emission settings appropriate for DiR (typically Ex 748 nm/Em 780 nm).

Techniques: In Vivo, Labeling, Injection, Fluorescence